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KMID : 0624620080410050363
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BMB Reports
2008 Volume.41 No. 5 p.363 ~ p.368
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Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M. smegmatis and E. coli
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Chattoraj Partho
Ganguly Tridib
Nandy Ranjan Kumar
Sau Subrata
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Abstract
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Two genes of temperate mycobacteriophage L5, namely, gp63 and gp64, were hypothesized to be toxic to M. smegmatis. An identical L5 gp64 ortholog (designated hlg1) was cloned from homoimmune mycobacteriophage L1 and characterized at length here. As expected, hlg1 affected the growth of M. smegmatis when overexpressed from a resident plasmid. HLG1 (the protein encoded by hlg1) in fact caused growth retardation of M. smegmatis and the region encompassing its 57-114 C-terminal amino acid residues was found indispensable for its growthretardation activity. Both nucleic acid and protein biosynthesis were severely impaired in M. smegmatis expressing HLG1. Interestingly, HLG1 also affected E. coli almost similarly. This putative delayed early lipoprotein did not participate in the lytic growth of L1. [BMB reports 2008; 41(5): 363-368]
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KEYWORD
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Delayed early gene, Growth retardation, Host lethal gene, Mycobacteriophage L1
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